TGFB1 ELISA Kit (Human)
Product Specifications
Reproducibility
The coefficient of variation of both intra-assay and inter-assay were less than 10%.
CAS Number
7732-18-5
Gene Name
Leptin
Gene Aliases
OB, OBS, LEPD
Gene ID
3953
Reactivity
Human
Target
This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development.
Type
ELISA Kit
Applications
ELISA
Detection Method
Colorimetric, OD450 nm
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Cell culture, supernates, serum, and plasma
Sensitivity
16 pg/ml
Homology
Human
Components
Components 2 vials human Leptin Standard lyophilized, 2000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human Leptin antibody 2 vials Streptavidin-HRP solution 4 bottles Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 2 vials human MCP-1 Standard lyophilized, 1000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human MCP-1 monoclonal antibody Components Components 2 vials human Leptin Standard lyophilized, 2000 pg/ml upon reconstitution 2 vials human Leptin Standard lyophilized, 2000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human Leptin antibody 2 vials concentrated Biotin-Conjugate anti-human Leptin antibody 2 vials Streptavidin-HRP solution 2 vials Streptavidin-HRP solution 4 bottles Standard /sample Diluent 4 bottles Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Substrate Solution 1 vial Stop Solution 1 vial Stop Solution 2 vials human MCP-1 Standard lyophilized, 1000 pg/ml upon reconstitution 2 vials human MCP-1 Standard lyophilized, 1000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human MCP-1 monoclonal antibody 2 vials concentrated Biotin-Conjugate anti-human MCP-1 monoclonal antibody
NCBI Gene Symbol
LEP
Gene Name URL
LEP
Nucleotide Accession Number
NM_000230
Frequently Asked Questions
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