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IL9 ELISA Kit (Human)

Product Specifications

Reproducibility

The coefficient of variation of both intra-assay and inter-assay were less than 10%.

CAS Number

7732-18-5

Gene Name

Interleukin 6

Gene Aliases

CDF, HGF, HSF, BSF2, IL-6, BSF-2, IFNB2, IFN-beta-2

Gene ID

3569

Reactivity

Human

Target

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants.

Type

ELISA Kit

Applications

ELISA

Detection Method

Colorimetric, OD450 nm

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Cell culture, supernates, serum, and plasma

Sensitivity

2 pg/ml

Homology

Human

Components

Components 2 vials human IL-6 Standard lyophilized, 1000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human IL-6 antibody 2 vials Streptavidin-HRP solution, 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 2 vials human IL-9 Standard lyophilized, 500 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human IL-9 monoclonal antibody Components Components 2 vials human IL-6 Standard lyophilized, 1000 pg/ml upon reconstitution 2 vials human IL-6 Standard lyophilized, 1000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human IL-6 antibody 2 vials concentrated Biotin-Conjugate anti-human IL-6 antibody 2 vials Streptavidin-HRP solution, 2 vials Streptavidin-HRP solution, 1 bottle Standard /sample Diluent 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Substrate Solution 1 vial Stop Solution 1 vial Stop Solution 2 vials human IL-9 Standard lyophilized, 500 pg/ml upon reconstitution 2 vials human IL-9 Standard lyophilized, 500 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human IL-9 monoclonal antibody 2 vials concentrated Biotin-Conjugate anti-human IL-9 monoclonal antibody

NCBI Gene Symbol

IL6

Gene Name URL

IL6

Nucleotide Accession Number

NM_000600

Frequently Asked Questions

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