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IL6 ELISA Kit (Human)

Product Specifications

Reproducibility

The coefficient of variation of both intra-assay and inter-assay were less than 10%.

CAS Number

7732-18-5

Gene Name

Interferon, gamma

Gene Aliases

IFG, IFI, IMD70

Gene ID

3459

Reactivity

Human

Target

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases.

Type

ELISA Kit

Applications

ELISA

Detection Method

Colorimetric, OD450 nm

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Cell culture, supernates, serum, and plasma

Sensitivity

8 pg/ml

Homology

Human

Components

Components 2 vials human IFN-gamma Standard lyophilized, 4000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human IFN-gamma antibody 2 vials Streptavidin-HRP solution 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 2 vials human IL-1alpha Standard lyophilized, 500 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human IL-1alpha monoclonal antibody Components Components 2 vials human IFN-gamma Standard lyophilized, 4000 pg/ml upon reconstitution 2 vials human IFN-gamma Standard lyophilized, 4000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human IFN-gamma antibody 2 vials concentrated Biotin-Conjugate anti-human IFN-gamma antibody 2 vials Streptavidin-HRP solution 2 vials Streptavidin-HRP solution 1 bottle Standard /sample Diluent 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Substrate Solution 1 vial Stop Solution 1 vial Stop Solution 2 vials human IL-1alpha Standard lyophilized, 500 pg/ml upon reconstitution 2 vials human IL-1alpha Standard lyophilized, 500 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human IL-1alpha monoclonal antibody 2 vials concentrated Biotin-Conjugate anti-human IL-1alpha monoclonal antibody

NCBI Gene Symbol

IFNG

Gene Name URL

IFNG

Nucleotide Accession Number

NM_000619

Frequently Asked Questions

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