L-Lysine 6-Oxidase, M. mediterranea

LodA is a novel enzyme (EC 1.4.3.20) that catalyzes the chemical reaction:L-lysine + O2 + H2O↔ 6-amino-2-oxohexanoate + NH3 + H2O2. It has antibacterial activity against a wide spectrum of Gram-positive and Gram-negative bacteria including nosocomial isolates of S.aureus and Pseudomonas sp. The antimicrobial activity is due to hydrogen peroxide generated by its lysine oxidase activity. It also has autotoxic activity. Involved in biofilm differentiation; responsible for cell death within microcolonies during biofilm development which is linked to the generation of a phenotypically diverse dispersal population and thus may play a role in colonization. The lysine oxidase is used in the enzymatic assay of L-lysine.