CaspGLOW™ Green Active Caspase-3/7 Staining Kit

Activation of caspases plays a central role in apoptosis. The CaspGLOW™ Green Active Caspase-3/7 Staining Kit provides a convenient means for detecting activated caspase-3/7 in living cells. The assay utilizes the caspase inhibitor DEVD-FMK conjugated to FITC (Green-DEVD-FMK) as the fluorescent in situ marker. Green-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to the activated caspase-3/7 in apoptotic cells. The green fluorescence label allows the direct detection of activated caspase-3/7 in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader. The caspase inhibitor, Z-VAD-FMK, is included in the kit as an additional control. It can be used to inhibit caspase activation in an induced culture.