Alpha Crosslaps (αCTx) (Human) ELISA Kit

CrossLaps (CTx) peptide [Glu-Lys-Ala-His-Asp-Gly-Gly-Arg], a part of the C-telopeptide of the alpha 1-chain of type I collagen of bone, is developed as a biochemical marker of bone turnover. The Alpha CrossLaps is intended for use as an indicator of degradation of non-isomerized bone collagen and may be used as an aid in the identification of skeletal metastases in patients with breast and prostate cancer. BioVision’s Alpha Crosslaps (αCTx) (Human) ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with αCTx. During the reaction, αCTx in the samples or standard competes with αCTx coated on the plate for binding to the anti- αCTx antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of αCTx. The concentration of αCTx in the samples can be calculated by comparing the OD of the samples to the standard curve.