Leukotriene B4 ELISA Kit

Leukotriene B4 (LTB4) is a pro-inflammatory mediator synthesized in myeloid cells from arachidonic acid. Leukotrienes are lipid mediators whose production is increased during inflammation. Leukotriene B4 stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator of inflammatory diseases and immunoregulation. BioVision’s LTB4 ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with LTB4. During the reaction, LTB4 in the samples or standard competes with LTB4 coated on the plate for binding to the anti-LTB4 antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of LTB4. The concentration of LTB4 in the samples can be calculated by comparing the OD of the samples to the standard curve.