METAP2 (Human) ELISA Kit

Methionine aminopeptidase (MetAP) is a bifunctional protein that plays a critical role in the regulation of post-translational processing and protein synthesis. MetAP function is essential for cell proliferation and viability. MetAP2 plays an important role in the development of different types of cancer. BioVision’s METAP2 (Human) ELISA Kit is based on the Competitive ELISA principle for the quantitative measurement of human METAP2 in serum, plasma and other biological fluids. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.