ToxOut™ Phase Extraction Endotoxin Removal Kit

Endotoxin is a lipopolysaccharide (LPS) complex located in the outer membrane of gram-negative bacteria. A single E.coli bacterium contains ~2 million LPS molecules. During experimental procedures, large amount of endotoxin is shed and can easily contaminate labware, buffers and downstream products. In vitro, endotoxin causes a variety of problems in cell-based research. In vivo, endotoxin may cause various side effects, including inflammatory response, organ failure or septic shock in host organisms. Therefore, it is critical to remove endotoxin from samples and products. BioVision‘s ToxOutTM Phase Extraction Endotoxin Removal Kit can effectively eliminate high concentration endotoxin in solutions containing proteins or pharmacologically important components down to <0.05 EU/ml without using toxic buffers, while maintaining protein recovery at >90%. BioVision‘s ToxOutTM Phase Extraction Endotoxin Removal Kit is based on Triton X-114 Phase Extraction (PE), which shows strong endotoxin removal capabilities. The residual Triton X-114 is further eliminated with our proprietary beads to achieve minimal detergent level in the final samples. The protein recovery is >90% depending on individual protein and typically greater than 95%.