Alpha-Mannosidase Activity Assay Kit (Fluorometric)

Alpha-Mannosidase (α-Man; EC 3.2.1.24) is a glycoside hydrolase involved in the cleavage of the alpha-mannose. α-Man can be applied in studies that determine the effects of mannose on specific molecules, such as recombinant proteins that are used for vaccine development. Lysosomal α-Man is essential for catabolizing the Asn-linked glycans of glycoproteins and is actively involved in maintaining the cellular homeostasis. α-Man deficiency results in Alpha-Mannosidosis, an acquired lysosomal storage disorder in which α-Man fails to break down mannose-containing oligosaccharides. These oligosaccharides, therefore, accumulate in lysosomes thereby leading to cellular malfunction and apoptosis. BioVision’s Alpha-Mannosidase Activity Assay Kit provides a facile, rapid way to monitor total α-Man activity in various biological samples. In this kit, α-Man cleaves a synthetic specific substrate and releases a fluorophore, which can be easily quantified (Ex/Em = 360/445 nm). The substrate is specific to α-Man and can differentiate from β-mannosidase. The assay is simple, sensitive and can detect as low as 1.0 µU of alpha mannosidase activity.