miRNA Extraction Kit

BioVision’s miRNA Extraction Kit is used to purify miRNA from cells and tissues. This kit is based on an improved guanidine thiocyanate- phenol one step method and elution of miRNA using spin columns. In this kit, the ribonucleases are inactivated and the genomic DNA, 18S and 28S RNAs are removed using spin columns. Cellular metabolite and proteins are removed by a series of elution-centrifugation steps. Subsequently, the microRNAs (including miRNA, snRNA, and other RNA less than 200 bp) are eluted from a second spin column in RNase-free water. The purified miRNA is ready for downstream applications including RNA sequencing, microarray, RT-PCR, Northern Blot etc.