Adeno-Associated Virus qPCR Quantification Kit

BioVision’s Adeno-Associated Virus qPCR Quantification Kit is used for the quantification of adeno-associated Virus. This kit employs a quick DNA extraction step from AAV particles, followed by a qPCR assay. The kit has demonstrated high sensitivity and specificity. Virus samples originating from both virus-producing cell lines or purified viral preparations can be used for determining the titer. Viral DNA is isolated from the sample using the Virus Lysis Buffer provided in the kit. Primers included in the Reagent Mix are then used with the extracted viral DNA, AAV STD1 and STD2 to determine the threshold cycle (Ct) values by qPCR. The Ct values are then used to calculate the viral titer of the adeno-associated virus samples.