DNA Library Prep Kit for IIlumina Sequencing

BioVision’s DNA Library Prep Kit for IIlumina Sequencing is used to efficiently convert (2 ng-500 ng) of DNA fragments into fully compatible libraries for Illumina Next Generation Sequencing (NGS) platforms. The sample preparation step involves the preparation of a DNA library by fragmentation, end repair, adenylation, ligation with adapter sequences and amplification. The DNA library is then loaded onto the chips for amplification and sequencing. The presence of adapter sequences allows the hybridization of the libraries to the sequencing chips and provides a universal priming site for sequencing primers. BioVision’s DNA Library Prep Kit contains index adapters needed for running multiple samples on a single Illumina flow cell as well as the magnetic beads for purification, which is required for the reaction cleanup after ligation and PCR steps. The entire procedure can be completed within 2 hours.