HAUS8 Antibody
HAUS8 antibody
Product Specifications
Product Name Alternative
HAUS8, HAUS augmin-like complex subunit 8, HEC1/NDC80-interacting centrosome-associated protein 1, Sarcoma antigen NY-SAR-48, rabbit anti-HICE1 Antibody
UniProt
Q9BT25
Reactivity
Human
Immunogen
Anti-HICE1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant full length Hice1 protein.
Target
HAUS8
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
38 mg/mL
Dilution
ELISA: 1:250,000, WB: 1:10,000
Purity
This product was adsorbed against GST from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with endogenous Hice1 protein. A BLAST analysis was used to suggest reactivity with Hice1 from human based on a 100% homology with the immunizing sequence. Expect reactivity with Hice1 from chimpanzee, Sumatran orangutan based on a 90% homology with the immunizing sequence. Cross-reactivity with Hice1 from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This antiserum has been tested for use in ELISA and western blotting using a full length recombinant Hice1 protein. Specific conditions for reactivity and detection of Hice1 should be optimized by the end user. Expect a band approximately ~45 kDa in size corresponding to Hice1 by Western Blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_219485
Host or Source
Rabbit
Preservative
0.01% (w/v) Sodium Azide
Isotype
Antiserum
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