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HAUS8 Antibody

HAUS8 antibody

Product Specifications

Product Name Alternative

HAUS8, HAUS augmin-like complex subunit 8, HEC1/NDC80-interacting centrosome-associated protein 1, Sarcoma antigen NY-SAR-48, rabbit anti-HICE1 Antibody

UniProt

Q9BT25

Reactivity

Human

Immunogen

Anti-HICE1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant full length Hice1 protein.

Target

HAUS8

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

38 mg/mL

Dilution

ELISA: 1:250,000, WB: 1:10,000

Purity

This product was adsorbed against GST from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with endogenous Hice1 protein. A BLAST analysis was used to suggest reactivity with Hice1 from human based on a 100% homology with the immunizing sequence. Expect reactivity with Hice1 from chimpanzee, Sumatran orangutan based on a 90% homology with the immunizing sequence. Cross-reactivity with Hice1 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This antiserum has been tested for use in ELISA and western blotting using a full length recombinant Hice1 protein. Specific conditions for reactivity and detection of Hice1 should be optimized by the end user. Expect a band approximately ~45 kDa in size corresponding to Hice1 by Western Blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_219485

Host or Source

Rabbit

Preservative

0.01% (w/v) Sodium Azide

Isotype

Antiserum

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