Monobromobimane

Monobromobimane (mBBr) is a cell-permeable, thiol-reactive fluorescent probe that is widely used to detect various thiol-containing biomolecules such as glutathione in cells. It reacts rapidly at physiological pH with available thiol groups, and generates a stable fluorescent signal. Monobromobimane can be used to evaluate or quantify a variety of compounds containing reactive sulfur or thiol groups, including H2S, glutathione, proteins, and nucleotides. The excitation and emission maxima for monobromobimane are 398 and 490 nm, respectively.