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GOT1 Antibody

GOT1 antibody

Product Specifications

Product Name Alternative

Sheep anti-Aspartate Transaminase Antibody, Aspartate aminotransferase 1 antibody, Aspartate aminotransferase cytoplasmic antibody, Aspartate aminotransferase cytosolic antibody, GIG 18 antibody, GIG18 antibody, Glutamate oxaloacetate transaminase 1 antibody

UniProt

P00503

Reactivity

Porcine

Immunogen

Aspartate Aminotransferase (AST) [Pig Heart]

Target

GOT1

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

96 mg/mL

Dilution

ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000

Purity

This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum, purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart]. Cross reactivity against Aspartate Aminotransferase (AST) from other tissues and species may occur but have not been specifically determined.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Aspartate Transaminase has been assayed against 1.0 ug of Aspartate Aminotransferase (AST) [Pig Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for this product.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_999092.1

Host or Source

Sheep

Preservative

0.01% (w/v) Sodium Azide

Isotype

Antiserum

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