REL Antibody
REL antibody
Product Specifications
Product Name Alternative
Rabbit anti-cRel Antibody, rabbit anti-NFkB Antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB, REL, c-Rel
UniProt
Q04864
Reactivity
Human
Immunogen
NFkB cRel peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) .
Target
REL
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Concentration
80 mg/ml
Dilution
ELISA: 1:5,000 - 1:25,000, Gel Supershift Assays: 1:500, WB: 1:500 - 1:3,000
Purity
This product was prepared from monospecific antiserum by delipidation and defibrination. Anti-NFkB cRel may react non-specifically with other proteins. Control peptide (orb343890) will compete only with the specific reaction of antiserum with the NFkB cRel subunit.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-NFkB cRel was tested by ELISA and by immunoblot. Anti-NFkB cRel is suitable for the detection by immunoblot of human NFkB cRel at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) . No reaction was observed against the analogous Mouse protein. This product was also tested in a gel supershift assay and found to be reactive against all cRel containing human NFkB complexes using 0.5 to 1.0 μl per assay.
Tested Applications
ELISA, Gel Supershift Assays, WB
NCBI Accession Number
NP_001278675.1
Host or Source
Rabbit
Preservative
0.01% (w/v) Sodium Azide
Isotype
Antiserum
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