NFKB1 Antibody

NFKB1 antibody

Product Specifications

Product Name Alternative

Rabbit Anti-NFKB p50 antibody, rabbit anti-NFKB1 antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB

UniProt

P19838

Reactivity

Human

Immunogen

Human NFkB p50 (NFKB1) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) .

Target

NFKB1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Concentration

80 mg/mL

Dilution

ELISA: 1:5,000 - 1:25,000, Gel Supershift Assays: 0.5 to 1.0 μl, WB: 1:500 - 1:2,000

Purity

This product was prepared from monospecific antiserum by delipidation and defibrination. Anti-Human NFkB p50 (NFKB1) may react non-specifically with other proteins. Control peptide (orb343887) will compete only with the specific reaction of antiserum with Human NFkB p50 (NFKB1) .

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This product was tested by immunoblot and ELISA. Anti-Human NFkB p50 (NFKB1) is suitable for the detection by immunoblot of Human NFkB p50 (NFKB1) and its precursor protein p105, and was found to be reactive against Human NFkB p50 (NFKB1) at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) . No reaction was observed against the analogous mouse protein. This product was also tested in a gel supershift assay and found to be reactive against p50:p50 homodimers and p:50:p65 heterodimers using 0.5 to 1.0 μl per assay.