Peroxidase Antibody
Peroxidase antibody
Product Specifications
Product Name Alternative
Rabbit anti-HRP, Peroxidase antibody, anti-HRP, Peroxidase antibody raised in rabbit, Rb-anti-HRP, Rb-anti-Peroxidase, Horseradish Peroxidase
Immunogen
Anti-Peroxidase antibody was produced by repeated immunization of rabbits with peroxidase isolated from horseradish.
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
90 mg/mL
Dilution
ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Purity
Anti-Peroxidase antibody was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Peroxidase [Horseradish]. Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.
Form
Lyophilized
Storage Conditions
Store anti-HRP at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Peroxidase antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. Note the buffer for this anti-peroxidase antibody is 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.6
Tested Applications
ELISA, IHC, WB
Host or Source
Rabbit
Preservative
None
Isotype
Antiserum
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