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ALP1 Antibody

ALP1 antibody

Product Specifications

Product Name Alternative

Rabbit anti-Alkaline Phosphatase Antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody

UniProt

P19111

Reactivity

Bovine

Immunogen

Alkaline Phosphatase [Calf intestine]

Target

ALP1

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

85 mg/mL

Dilution

ELISA: 1:4,000 - 1:20,000, WB: 1:500 - 1:2,000

Purity

This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Alkaline Phosphatase [Calf intestine]. Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined. No reaction was observed against the bacterial form of the enzyme.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Alkaline Phosphatase has been assayed against 1.0 ug of Alkaline Phosphatase [Calf intestine] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.

Tested Applications

ELISA, WB

Host or Source

Rabbit

Preservative

None

Isotype

Antiserum

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