ORAI1 Antibody

Anti-ORAI-1 antibody is directed against the human ORAI-1. The product was prepared from monospecific antiserum by delipidation and defibrination. The antiserum was further cross-absorbed against MBP by chromatography. Cross-reactivity with ORAI1 from other sources have not been determined.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Anti-ORAI-1 has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect bands approximately 32.7 kDa by western blotting in the appropriate cell lysate or extract.