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Pecam1 Antibody

CD31 antibody

Product Specifications

Product Name Alternative

Rabbit anti-CD31 antibody, rabbit anti-PECAM-1 antibody, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD-31 Antibody, PECAM1 Antibody, CD31/PECAM1 Antibody

UniProt

Q3SWT0

Reactivity

Human, Mouse

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of rat CD31 protein.

Target

Pecam1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Concentration

90 mg/mL

Dilution

ELISA: 1:20,000, IHC: 4μg/mL, WB: 1:500

Purity

CD31 PECAM1 antibody is directed against rat CD31 protein. The product is delipidated and defibrinated antiserum. A BLAST analysis was used to suggest cross-reactivity with CD31 from rat, human, and mouse based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

CD31 antibody has been tested for use in ELISA, immunohistochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80 kDa in size corresponding to CD31 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_113779.1

Host or Source

Rabbit

Preservative

0.01% (w/v) Sodium Azide

Isotype

IgG

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