Pecam1 Antibody
CD31 antibody
Product Specifications
Product Name Alternative
Rabbit anti-CD31 antibody, rabbit anti-PECAM-1 antibody, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD-31 Antibody, PECAM1 Antibody, CD31/PECAM1 Antibody
UniProt
Q3SWT0
Reactivity
Human, Mouse
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of rat CD31 protein.
Target
Pecam1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Concentration
90 mg/mL
Dilution
ELISA: 1:20,000, IHC: 4μg/mL, WB: 1:500
Purity
CD31 PECAM1 antibody is directed against rat CD31 protein. The product is delipidated and defibrinated antiserum. A BLAST analysis was used to suggest cross-reactivity with CD31 from rat, human, and mouse based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
CD31 antibody has been tested for use in ELISA, immunohistochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80 kDa in size corresponding to CD31 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_113779.1
Host or Source
Rabbit
Preservative
0.01% (w/v) Sodium Azide
Isotype
IgG
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