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KIF5B Antibody

KIF5B antibody

Product Specifications

Product Name Alternative

Rabbit anti-Kinesin 1 Antibody, Conventional kinesin heavy chain, Ubiquitous kinesin heavy chain, KNS, KNS1, Kinesin-1 heavy chain, UKHC, Anti-Kinesin-1 antibody, kinesin 1 antibody, kin1, kin-1, KIF5, KHC, Kinesin motor domain

UniProt

P33176

Reactivity

Human

Immunogen

Anti-Kinesin-1 was prepared from whole rabbit serum produced by repeated immunizations with a truncated kinesin-1 construct expressed in E. coli corresponding to human kinsesin-1protein.

Target

KIF5B

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

77 mg/mL

Dilution

ELISA: 1:10000, WB: 1:100-1:500

Purity

Anti-Kinesin-1 is directed against the human kinesin-1 protein. The product was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest reactivity with human. Cross-reactivity with kinesin 1 from other sources have not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Kinesin-1 antibody has been tested for use in SDS, western blot, and immunohistochemistry. For western blots expect a band of approximately 72 kDa in size corresponding to truncated kinesin-1 protein. Specific conditions for reactivity should be optimized by the end user.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_004512.1

Host or Source

Rabbit

Preservative

0.01% (w/v) Sodium Azide

Isotype

Antiserum

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