ANAPC11 Antibody

Rabbit anti-Apc11 Antibody, Anaphase promoting complex subunit 11 homolog antibody, APC11 anaphase promoting complex subunit 11 homolog antibody, Cyclosome subunit 11 antibody, Hepatocellular carcinoma associated RING finger protein antibody, HSPC 214 antibody, Apc

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 76-84 of Human APC11 (C-terminal) coupled to KLH.

This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human and mouse APC11. Cross reactivity may also occur with APC11 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and ROC2.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody reacts with human APC11 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others) . Coimmunoprecipitation of related proteins (APC2) does occur. A 9.8 kDa band corresponding to human APC11 is detected. Most cell lines or tissues expressing APC11 can be used as a positive control. Researchers should determine optimal titers for other applications.