CUL2 Antibody

Anti-Cul2 was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminus region near amino acids 720-745 of Human Cul2 coupled to KLH.

Antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 2. Cross reactivity with Cul2 from other sources has not been determined.

Store Cul2 antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Cul2 has been tested by immunohistochemistry. This antibody reacts with human Cul2 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated product and protein from cell lysates (using HeLa or NIH-3T3) . An 86.9 kDa band corresponding to human Cul2 is detected. Most cell lines expressing Cul2 can be used as a positive control. Researchers should determine optimal titers for other applications.