TetX Antibody

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a polyhistidine-tagged TTFC fusion protein expressed in E. coli corresponding to full length TTFC.

This antibody is directed against the C fragment of Tetanus toxin derived from Clostridium tetani. The product was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest cross reactivity with Clostridium tetani. Cross-reactivity with TTFC from other sources have not been determined.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

This antibody has been tested for use in ELISA and western blot. For western blots expect a band of approximately 52 kDa in size corresponding to full-length TTFC. Specific conditions for reactivity should be optimized by the end user.