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TetX Antibody

TetX antibody

Product Specifications

Product Name Alternative

Rabbit anti-Tetanus Toxin Fragment C Antibody , TTfC, tentoxylysin, tetX

UniProt

P04958

Reactivity

Bacteria

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a polyhistidine-tagged TTFC fusion protein expressed in E. coli corresponding to full length TTFC.

Target

TetX

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

90 mg/mL

Dilution

ELISA: 1:50,000 - 1:250,000, WB: 1:1,000 - 1:40,000

Purity

This antibody is directed against the C fragment of Tetanus toxin derived from Clostridium tetani. The product was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest cross reactivity with Clostridium tetani. Cross-reactivity with TTFC from other sources have not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This antibody has been tested for use in ELISA and western blot. For western blots expect a band of approximately 52 kDa in size corresponding to full-length TTFC. Specific conditions for reactivity should be optimized by the end user.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_783831.1

Host or Source

Rabbit

Preservative

0.01% (w/v) Sodium Azide

Isotype

Antiserum

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