Angpt1 Antibody

Rabbit anti-Angiopoietin 1 Antibody, rabbit anti-ANG1 antibody, AGP 1 antibody, AGP1 antibody, AGPT antibody, ANG 1 antibody, Angiopoietin1 antibody, ANGPT 1 antibody, ANGPT1 antibody, KIAA0003 antibody

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to a N-terminus region near aa 15-45 of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.

Anti-Angiopoietin 1 is directed against mouse angiopoietin-1 and shows no reactivity with mouse angiopoietin-2. This reagent cross-reacts with human angiopoietin 1. Partially cross reactivity is noted with human angiopoietin 2. This product was prepared from monospecific antiserum by a delipidation and defibrination.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Anti-Antiopoietin-1 Antiserum has been tested in ELISA, western blot, immunohistochemistry, and immunofluorescence and is suitable for other antibody based assays. A 1:500 dilution is recommended for western blotting. The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum. This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in a very clean and strong signal. Both Ang-1 and Ang-2 proteins have predicted molecular weights of approximately 57 kDa and appear on western blots close to their predicted molecular weights. In some instances additional bands may be seen at approximately 75 kDa which represent highly glycosylated forms of the protein that migrate at a higher apparent molecular weight.