Angpt2 Antibody

Rabbit anti-Angiopoietin 2 Antibody, rabbit anti-ANG2 antibody, AGPT 2 antibody, Agpt2 antibody, ANG 2 antibody, ANG2 antibody, Angiopoietin 2a antibody, Angiopoietin 2B antibody, Angiopoietin2 antibody, ANGPT 2 antibody, ANGPT2 antibody, Tie2 ligand antibody

Anti-Angiopoietin 2 Antiserum was prepared by repeated immunizations with a synthetic peptide, corresponding to a region near the N-terminus of mouse angiopoietin-2 protein, conjugated to KLH using maleimide.

Angiopoietin 2 Antibody antiserum is directed against mouse angiopoietin-2 and shows no reactivity with angiopoietin-1 from mouse-derived proteins. This product was prepared from monospecific antiserum by a delipidation and defibrination. This reagent cross-reacts with human angiopoietin-2. Partially cross-reactivity is noted with human angiopoietin-1.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Angiopoietin 2 Antibody is tested in WB, ICC, and IHC. Angiopoietin 2 Antibody is suitable for western blotting and other antibody based assays. A 1:500 dilution is recommended for western blotting. Both Ang-1 and Ang-2 proteins have predicted molecular weights of approximately 57 kDa and appear on western blots close to their predicted molecular weights. In some instances additional bands may be seen at approximately 75 kDa which represent highly glycosylated forms of the protein that migrate at a higher apparent molecular weight. The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum. This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in a very clean, strong signal.