AKT1 Antibody

Rabbit anti-AKT antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT Serine/Threonine Kinase 1, AKT 1 Antibody, AKT-1 Antibody

AKT Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A corresponding to the C-terminus (460-480) of human AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling. A BLAST analysis was used to suggest reactivity with this protein from rat, mouse, and chicken based on 100% homology for the immunogen sequence.

This product was prepared from monospecific antiserum by a delipidation and defibrination. Pan Anti-AKT Antibody reacts with the AKT from human tissues. Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT.

Store Anti-Akt antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-AKT Antibody has been tested in Western Blot, Immunohistochemistry (Formalin-fixed paraffin-embedded sections), and Immunofluorescence (paraformaldehyde-fixed primary cardiomyocyte cultures) . Expect a band at ~55.7kDa in 3T3 whole cell lysate or other appropriate cell lysates or tissues in western blot. Although not tested, this antibody would be useful in flow cytometry. Researchers should determine optimal titers for applications that are not stated below.