CHUK Antibody

CHUK antibody

Product Specifications

Product Name Alternative

Rabbit anti-IKK alpha Antibody, Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase alpha, IkBKA, IKK-alpha, IKK-A, IkappaB kinase, IKKA, TCF16

UniProt

O15111

Reactivity

Human, Mouse, Rat

Immunogen

IKK a peptide corresponding to the highly conserved C-terminus region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) .

Target

CHUK

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Concentration

80 mg/mL

Dilution

ELISA: 1:5,000 - 1:25,000, IHC: 1:500, WB: 1:500 - 1:3,000

Purity

Anti-IKKα was prepared from monospecific antiserum by delipidation and defibrination. Anti- IKK a may react non-specifically with other proteins. Control peptide (orb343885) will compete only with the specific reaction of antiserum with the IKK a subunit.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-IKKα antibody was tested by immunoblot and found to be reactive against IKK alpha at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) . Anti-IKKa is suitable for the detection by immunoblot of human, mouse and rat IKKa showing an 85 kDa band. This product is tested in IHC.