CDKN2A Antibody

Rabbit anti-p16 Antibody, Cyclin-dependent kinase inhibitor 2A isoforms 1/2/, Cyclin-dependent kinase 4 inhibitor A, CDK4I, p16-INK4a, p16INK4A, p16-INK4, Multiple tumor suppressor 1, MTS-1, CDKN2, MTS1

This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a p16 protein from human tissue. Cross reactivity may occur with p15 when immunoprecipitating in vitro translated product but not in immunoblots. Potentially cross reacts with p15.5.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This product was tested by immunoblot and found to be reactive against p16 from cell lysates and E.coli produced fusion proteins at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) . Anti-p16 is suitable for the detection by immunoblot of human p16. This antisera is also suitable for immunoprecipitation of in vitro translated protein and cell lysates (Saos-2, 3T3-L1 weakly) and will co-precipitate associated proteins including p15 and potentially p15.5. This product was tested in IHC.