CCND1 Antibody
CCND1 antibody
Product Specifications
Product Name Alternative
Rabbit anti-cyclin D1 antibody, G1/S-specific cyclin-D1, PRAD1 oncogene, BCL-1 oncogene
UniProt
P24385
Reactivity
Human
Immunogen
Anti-Cyclin D1 was produced by repeated immunizations of full length fusion protein corresponding to the human gene sequence.
Target
CCND1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Concentration
85 mg/mL
Dilution
ELISA: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:500 - 1:1,000
Purity
This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a 40-45 kDa Cyclin D1 protein from human, rat and mouse tissue. No reaction was observed against other related cyclins. Cross reactivity with Cyclin D1 from other species may also occur.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 34 kDa in size corresponding to Cyclin D1 by western blotting in the appropriate cell lysate or extract. MCF7 may be used as a positive control. Anti-Cyclin D1 is suitable for the detection by immunoblot of human, rat and mouse Cyclin D1.
Tested Applications
ELISA, IP, WB
NCBI Accession Number
NP_444284.1
Host or Source
Rabbit
Preservative
0.01% (w/v) Sodium Azide
Isotype
Antiserum
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