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CCND1 Antibody

CCND1 antibody

Product Specifications

Product Name Alternative

Rabbit anti-cyclin D1 antibody, G1/S-specific cyclin-D1, PRAD1 oncogene, BCL-1 oncogene

UniProt

P24385

Reactivity

Human

Immunogen

Anti-Cyclin D1 was produced by repeated immunizations of full length fusion protein corresponding to the human gene sequence.

Target

CCND1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Concentration

85 mg/mL

Dilution

ELISA: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:500 - 1:1,000

Purity

This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a 40-45 kDa Cyclin D1 protein from human, rat and mouse tissue. No reaction was observed against other related cyclins. Cross reactivity with Cyclin D1 from other species may also occur.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 34 kDa in size corresponding to Cyclin D1 by western blotting in the appropriate cell lysate or extract. MCF7 may be used as a positive control. Anti-Cyclin D1 is suitable for the detection by immunoblot of human, rat and mouse Cyclin D1.

Tested Applications

ELISA, IP, WB

NCBI Accession Number

NP_444284.1

Host or Source

Rabbit

Preservative

0.01% (w/v) Sodium Azide

Isotype

Antiserum

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