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PRC1 Antibody

PRC1 antibody

Product Specifications

Product Name Alternative

Rabbit anti-Carboxypeptidase Y antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody

UniProt

P00729

Reactivity

Yeast

Immunogen

Carboxypeptidase Y [Baker’s Yeast]

Target

PRC1

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

90 mg/mL

Dilution

ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000

Purity

This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Carboxypeptidase Y [Baker’s Yeast]. Cross reactivity against Carboxypeptidase Y from other tissues and species may occur but have not been specifically determined.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Carboxypeptidase Y has been tested by western blot and is suitable to be assayed against 1.0 μg of Carboxypeptidase Y [Baker’s Yeast] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_014026.1

Host or Source

Rabbit

Preservative

0.01% (w/v) Sodium Azide

Isotype

Antiserum

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