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Peroxidase (Goat) Antibody

Peroxidase antibody

Product Specifications

Product Name Alternative

HRP, Horseradish peroxidase, Goat anti-peroxidase antibody, anti-HRP

UniProt

Q42517

Immunogen

Peroxidase [Horseradish]

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Concentration

45.0 mg/mL

Dilution

ELISA: 1:9,000 - 1:40,000, WB: 1:900 - 1:4,000

Purity

Anti-Peroxidase (Horseradish) (goat) antibody was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Peroxidase [Horseradish]. Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.

Form

Lyophilized

Storage Conditions

Store anti-HRP at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Peroxidase (Horseradish) (Goat) Antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Peroxidase [Horseradish] in a standard ELISA using Alkaline Phosphatase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and pNPP as a substrate for 30 minutes at room temperature. A working dilution of 1:9,000 to 1:40,000 of the reconstitution concentration is suggested for this product.

Tested Applications

ELISA, WB

Host or Source

Goat

Preservative

None

Isotype

Antiserum

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