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ChoA Antibody

ChoA antibody

Product Specifications

Product Name Alternative

Goat anti-Cholesterol Oxidase Antibody, choA antibody, choD antibody, choM antibody, choS antibody, COD antibody

UniProt

P12676

Reactivity

Bacteria

Immunogen

Cholesterol Oxidase [Streptomyces]

Target

ChoA

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

90 mg/mL

Dilution

ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000

Purity

This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Cholesterol Oxidase [Microorganism]. Cross reactivity against Cholesterol Oxidase from other sources is unknown.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Cholesterol Oxidase has been tested by western blot and is suitable to be assayed against 1.0 ug of Cholesterol Oxidase [Microorganism] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:40,000 of the reconstitution concentration is suggested for this product.

Tested Applications

ELISA, IP, WB

Host or Source

Goat

Preservative

0.01% (w/v) Sodium Azide

Isotype

Antiserum

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