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Alpha-1-Trypsin Antibody

Alpha 1 Antitrypsin antibody

Product Specifications

Product Name Alternative

Goat anti-A1AT antibody, AAT antibody, Alpha 1 antiproteinase antibody, goat anti-Alpha 1 antitrypsin antibody, Alpha 1 protease inhibitor antibody, goat anti-α-1-Anti-Trypsin Antibody

UniProt

P01009

Reactivity

Human

Immunogen

A1-Anti-Trypsin [Human Plasma]

Target

SERPINA1

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

90 mg/mL

Dilution

ELISA: 1:20,000 - 1:100,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000

Purity

This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified a1-Anti-Trypsin [Human Plasma]. Cross reactivity against a1-Anti-Trypsin from other sources is unknown.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Alpha-1-AntiTrypsin has been tested by western blot and is suitable to be assayed against 1.0 ug of Alpha-1-Anti-Trypsin [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:30,000 of the reconstitution concentration is suggested for this product.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_000286.3

Host or Source

Goat

Preservative

0.01% (w/v) Sodium Azide

Isotype

Antiserum

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