Lysine Acetylated Antibody Biotin Conjugated
Lysine Acetylated antibody (Biotin)
Product Specifications
Product Name Alternative
Acetyl Lysine antibody, Acetylated lysine antibody, Lysine antibody
Immunogen
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with acetylated lysine conjugated KLH.
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Pharmacology & Drug Discovery
Concentration
1 mg/mL
Dilution
ELISA: 1:5,000, WB: 1:1,000-1:5,000
Purity
Anti-AcK was prepared from monospecific antiserum by immunoaffinity chromatography using acetylated lysine peptide coupled to agarose followed by conjugation to biotin and subsequent purification. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Biotin. Reactivity is specific for acetylated lysine.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
This affinity-purified antibody is suitable for use in ELISA and western blotting assays. The antibody reacts specifically with acetylated lysine residues. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.
Tested Applications
ELISA, WB
Host or Source
Rabbit
Preservative
Preservative: None. Stabilizer: 50% (v/v) Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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