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MAP2K2 Antibody (HRP)

MEK2 antibody (Peroxidase)

Product Specifications

Product Name Alternative

Rabbit anti-MEK2 antibody peroxidase conjugation, HRP conjugated rabbit anti-MEK2 antibody, Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, MAP2K2, MEK, MEK 2, MKK2, PRKMK2, CFC4, MEK-2, ERK activator kinase 2, MAPK/ERK kinase 2

UniProt

P36507

Reactivity

Human, Mouse, Rat

Immunogen

Anti-MEK2 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH.

Target

MAP2K2

Clonality

Polyclonal

Conjugation

HRP

Field of Research

Signal Transduction

Concentration

1.0 mg/mL

Dilution

ELISA: 1:40,000, WB: 1μg/mL

Purity

This HRP conjugated affinity purified antibody is directed against human MEK2 protein. Anti-MEK2 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity. Cross reactivity is expected to occur with human, mouse and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK1 isoform.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-MEK 2 (RABBIT) antibody is suitable for use in ELISA and Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 44 kDa.

Tested Applications

ELISA, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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