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MAPK3 Antibody (HRP)

ERK1/2 antibody (Peroxidase)

Product Specifications

Product Name Alternative

Rabbit anti-ERK-1 Antibody Peroxidase conjugation, HRP conjugated rabbit anti-ERK-1 Antibody, MAPK3, ERK 1, ERK1, PRKM3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, microtubule-associated protein 2 kinase, p44-ERK1

UniProt

P27361

Reactivity

Human, Mouse, Rat

Immunogen

Anti-ERK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH.

Target

MAPK3

Clonality

Polyclonal

Conjugation

HRP

Field of Research

Cell Biology

Concentration

1.0 mg/mL

Dilution

ELISA: 1:40,000, WB: 1μg/mL

Purity

This HRP conjugated affinity purified antibody is directed against human ERK1 protein. Anti-ERK1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity. Cross reactivity is expected to occur with human, mouse and rat based on sequence identity of the peptide immunogen. This antibody does not react with the ERK2 isoform.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-ERK 1 (RABBIT) antibody is suitable for use in ELISA and Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43 kDa.

Tested Applications

ELISA, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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