HIST2H3C Antibody

Anti-Histone H3 [ac Lys9/phospho Ser10] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide with a acetylation surrounding Lysine 9 and a phosphorylation surrounding serine 10 of human Histone H3.

Anti-Histone H3 K9Ac/pS10 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3. Cross-reactivity with other sources has not been determined.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Histone H3 K9-Ac/pS10 antibody is tested for Western Blot, Dot Blot, and Immunocytochemistry/Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15 kDa corresponding to Histone H3 protein by Western Blotting in NIH-3T3 histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals.