What is the catalog number for HIST2H3C Antibody?

The catalog number for HIST2H3C Antibody is orb420432.

What sizes are available for HIST2H3C Antibody?

HIST2H3C Antibody is available in the following size(s): 50 µg.

Is HIST2H3C Antibody in stock?

HIST2H3C Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

HIST2H3C Antibody

Histone H3 K27me3 antibody

Product Specifications

Product Name Alternative

Rabbit anti-Histone H3 trimethyl Lys27 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K27me3

UniProt

Q71DI3

Reactivity

Human, Mouse, Rat

Immunogen

Histone H3 [Trimethyl Lys27] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the K27me3 site of human Histone H3.

Target

HIST2H3C

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Concentration

0.93 mg/ml

Dilution

ELISA: 1: 10,000, IF: 1:100, IP: 1:100, WB: 1:1000

Purity

Anti-Histone H3 [Trimethyl Lys27] was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, rat, and C. elegans based on 100% sequence homology. Cross-reactivity with Histone H3 [Lys27 me3] from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Histone H3 [Trimethyl Lys27] antibody is tested for Dot Blot, IF, and Western Blot. This antibody is useful for ELISA and immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4kDa corresponding to the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals.