HIST2H3C Antibody
Histone H3 K27me3 antibody
Product Specifications
Product Name Alternative
Rabbit anti-Histone H3 trimethyl Lys27 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K27me3
UniProt
Q71DI3
Reactivity
Human, Mouse, Rat
Immunogen
Histone H3 [Trimethyl Lys27] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the K27me3 site of human Histone H3.
Target
HIST2H3C
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Concentration
0.93 mg/ml
Dilution
ELISA: 1: 10,000, IF: 1:100, IP: 1:100, WB: 1:1000
Purity
Anti-Histone H3 [Trimethyl Lys27] was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, rat, and C. elegans based on 100% sequence homology. Cross-reactivity with Histone H3 [Lys27 me3] from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Histone H3 [Trimethyl Lys27] antibody is tested for Dot Blot, IF, and Western Blot. This antibody is useful for ELISA and immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4kDa corresponding to the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals.
Tested Applications
DOT, ELISA, IF, IP, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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