HIST2H3C Antibody
Histone H3 K56me1 antibody
Product Specifications
Product Name Alternative
Rabbit anti-Histone H3 monomethyl Lys56 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K56me1
UniProt
Q71DI3
Reactivity
Human, Mouse
Immunogen
Histone H3 [Monomethyl Lys56] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 56 of human Histone H3.
Target
HIST2H3C
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Concentration
1.1 mg/mL
Dilution
ChIP: 2-5μg/million cells, IHC: 1:20-1:100, IF: 1:20-1:100, WB: 1μg/mL
Purity
Anti-Histone H3 [Monomethyl Lys56] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Histone H3 [Monomethyl Lys56] antibody is tested for Western Blot, Immunofluorescence, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals.
Tested Applications
ChIP, DOT, IF, IHC, Multiplex Assay, WB
NCBI Accession Number
NP_001005464
Host or Source
Rabbit
Preservative
Preservative: 0.05% (w/v) Sodium Azide. Stabilizer: 30% Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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