What is the catalog number for HIST2H3C Antibody?

The catalog number for HIST2H3C Antibody is orb420418.

What sizes are available for HIST2H3C Antibody?

HIST2H3C Antibody is available in the following size(s): 50 µg.

Is HIST2H3C Antibody in stock?

HIST2H3C Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Is there an additional fee for hazardous or dry ice shipping of this product?

Yes, if this product requires dry ice or is classed as hazardous for transport, the additional fee will be explicitly shown in your quotation before you confirm the order, so there are no surprise charges later.

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

HIST2H3C Antibody

Histone H3 K37me2 antibody

Product Specifications

Product Name Alternative

Rabbit anti-Histone H3 dimethyl Lys37 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87782, MGC87783, H3K37me2

UniProt

Q71DI3

Reactivity

C. elegans, Human, Mouse

Immunogen

Histone H3 [Dimethyl Lys37] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 37 of human Histone H3.

Target

HIST2H3C

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Concentration

0.69 mg/mL

Dilution

ChIP: 2-5μg/million cells, IHC: 1:100, IF: 1:100, WB: 1:500-1:2000

Purity

Anti-Histone H3 [Dimethyl Lys37] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Histone H3 [Dimethyl Lys37] antibody is tested for Western Blot, Immunofluorescence, and Dot Blot. This antibody is useful for Chromatin Immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals.