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HIST2H3C Antibody

Histone H3 K37me1 antibody

Product Specifications

Product Name Alternative

Rabbit anti-Histone H3 monomethyl Lys37 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87782, MGC87783, H3K37me1

UniProt

Q71DI3

Reactivity

C. elegans, Human, Mouse

Immunogen

Histone H3 [Monomethyl Lys37] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic methylated peptide surrounding Lysine 37 of human Histone H3.

Target

HIST2H3C

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Concentration

1.0 mg/mL

Dilution

WB: 1μg/mL

Purity

Anti-Histone H3 [Monomethyl Lys37] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Histone H3 [Monomethyl Lys37] antibody is tested for Bot Blot, Western Blot, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Anti-Histone H3 is also useful for Dot Blot. Epi-Plus antibody production in collaboration with Novus Biologicals.

Tested Applications

DOT, IF, Multiplex Assay, WB

NCBI Accession Number

NP_001005464

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 30% Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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