What is the catalog number for HIST2H3C Antibody?

The catalog number for HIST2H3C Antibody is orb420395.

What sizes are available for HIST2H3C Antibody?

HIST2H3C Antibody is available in the following size(s): 50 µg.

Is HIST2H3C Antibody in stock?

HIST2H3C Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

HIST2H3C Antibody

Histone H3 R8me2s antibody

Product Specifications

Product Name Alternative

Rabbit anti-Histone H3 Sym-dimethyl Arg8 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3R8me2s

UniProt

Q71DI3

Reactivity

C. elegans, Human

Immunogen

Histone H3 [Sym-dimethyl Arg8] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic symmetric dimethylated peptide surrounding Arginine 8 of human Histone H3.2.

Target

HIST2H3C

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Concentration

0.52mg/mL

Dilution

ChIP: 2-5μg/million cells, WB: 2μg/mL

Purity

Anti-Histone H3 [Sym-dimethyl Arg8] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Histone H3 [Sym-dimethyl Arg8] antibody is tested for Western Blot, Chromatin Immunoprecipitation and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals.