RB1 Antibody

Retinoblastoma-associated protein, p105-Rb, pRb, pp110, RB Transcriptional Co-repressor, Protein Phosphatase 1 Regulatory Subunit 130, Prepro-Retinoblastoma-Associated Protein, Retinoblastoma, P105-Rb, Retinoblastoma Susceptibility Protein, Retinoblastoma-Associated Protein, Osteosarcoma, PPP1R130, OSRC

Anti-Retinoblastoma [Monomethyl Lys860] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a methylated and acetylated synthetic peptide surrounding lysine 860 of human RB1 conjugated to Keyhole Limpet Hemocyanin (KLH) .

This affinity purified antibody is directed against human Retinoblastoma. This product was affinity purified from monospecific antiserum by immunoaffinity purification. This antibody reacts with human RB1 surrounding aa860 and is specific for K860 Me1. Blast analysis of the sequence of the immunogen shows 100% identity with human and 92% identity to rat and mouse.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Anti-Retinoblastoma K860 Me1 Antibody was tested for use in ELISA, Dot Blot, and Western Blot. Expect a band at approximately 106.1kDa in appropriate cell lysates or tissues. Although not tested, expect reactivity in immunoprecipitation and immunohistochemistry.