TP53 Antibody

Rabbit anti-p53 Ac-Lys305 antibody, rabbit anti-p53 K305ac antibody, cellular tumor antigen p53, tumor suppressor p53, phosphoprotein p53, Antigen NY-CO-13, TP53

Anti-p53 K305 Ac antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 305 of human p53.

Anti-p53 K305 Ac antibody is directed against human p53 protein. p53 K305 Ac Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis used to suggest reactivity with this protein from human based on 100% homology for the immunizing sequence.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

P53 K305 Ac Antibody has been tested by dot blot and western blotting and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~43.6 kDa in size corresponding to p53 by western blotting in the appropriate cell lysate or extract.